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KMID : 0613820120220060837
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Journal of Life Science
2012 Volume.22 No. 6 p.837 ~ p.843
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Comparison of Endo-, Exo-Cellular Enzyme Activity for New Strains of Hypsizygus marmoreus
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Lee Chang-Yun
Song Ho-Sung
Ro Hyeon-Su
Woo Ju-Ri
You Young-Hyun
Kim Jong-Guk
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Abstract
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This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the 1st cultivation experiment, and selected six strains from one of 58 strains through the 2nd cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of α-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than α-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of α-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of β-glucosidase and chitinase is low.
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KEYWORD
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Hypsizygus marmoreus, α-amylase, CMCase, enzyme activity, new cultivars
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